2026-06-01T02:01:51Z https://icatplus.esrf.fr/oaipmh/request

oai:icatplus.esrf.fr:inv/2119591232 2025-06-02T06:00:29.673Z

4.2 EI 10.15151/ESRF-ES-2119591232 Catherine CURIE Catherine Curie 0000-0002-8884-1175 Catherine CURIE Catherine Curie 0000-0002-8884-1175 Tou Cheu XIONG Tou Cheu Xiong 0000-0002-3334-0939 Tou Cheu XIONG Tou Cheu Xiong 0000-0002-3334-0939 Hiram CASTILLO Hiram Castillo Michel GABRIEL SGARBIERO MONTANHA Gabriel Sgarbiero Montanha 0000-0003-1375-2351 GABRIEL SGARBIERO MONTANHA Gabriel Sgarbiero Montanha 0000-0003-1375-2351 Alexandra LESKOVA Alexandra Leskova 0000-0003-4587-2550 Alexandra LESKOVA Alexandra Leskova 0000-0003-4587-2550 Example Institute 2028 Data from large facility measurement 2025-05-29T06:00:00Z/2025-06-02T06:00:00Z 2025-06-02T06:00:00Z I CC-BY-4.0 Manganese (Mn) plays a crucial role in plants in photosynthesis, synthesis of antioxidants, and cell wall compounds. Efficient transport of Mn within and between cells, mediated by specific transporters, is essential for proper cellular function. Our research has revealed that the movement of Mn from the outer cell layers to the inner embryos in plant seeds relies on the NRAMP2 transporter, which is located on an intracellular organelle called the trans-Golgi network (manuscript in prep). Disruption of this transport mechanism results in altered zinc contents, seeds germination is susceptibility to seed-transmitted pathogens. A critical question remains: how does a transporter situated in an intracellular organelle facilitate cell-to-cell transport of Mn? We propose to map the localization of manganese and zinc in the developing seeds of wild type and nramp2 mutants at cellular and subcellular resolutions. This research could significantly advance the field of seed biofortification.