2026-07-27T18:45:49Z https://icatplus.esrf.fr/oaipmh/request

oai:icatplus.esrf.fr:inv/2116854002 2025-05-30T06:00:17.281Z

4.2 EI 10.15151/ESRF-ES-2116854002 Anne-Claire DHAUSSY Anne-Claire Dhaussy Anne-Claire DHAUSSY Anne-Claire Dhaussy Mohamed MEZOUAR Mohamed Mezouar 0000-0001-5336-544X Nicolas COQUELLE Nicolas Coquelle Nicolas COQUELLE Nicolas Coquelle Max GERIN Max Gerin Eric GIRARD Eric Girard 0000-0002-5758-6095 Eric GIRARD Eric Girard 0000-0002-5758-6095 Example Institute 2028 Data from large facility measurement 2025-05-27T06:00:00Z/2025-05-30T06:00:00Z 2025-05-30T06:00:00Z I CC-BY-4.0 High pressure macromolecular crystallography (HPMX) is an ideal technique to trap high-energy transient conformers of proteins that are essential for biological activity and/or for interaction with partners. Using a diamond anvil cell (DAC) and collecting data at room temperature (RT) ensures that the pressure/temperature of the sample environment is precisely defined and controlled. For proteins, this usually requires large enough crystals to collect a complete data set at high resolution, hopefully radiation damage free. However, growing such crystals remain a challenge. Serial crystallography offers the possibility to collect diffraction data from micron-sized crystals at RT. Indeed, only a single diffraction frame is recorded from a small single crystal, therefore spreading the required X-ray dose to reconstruct a complete dataset over a very large number of micron-sized crystals. we want to evaluate the feasibility to combine SX and DAC techniques to push forward HPMX.