2026-06-01T16:09:11Z https://icatplus.esrf.fr/oaipmh/request

oai:icatplus.esrf.fr:inv/2108132504 2025-05-13T06:02:05.094Z

4.2 EI 10.15151/ESRF-ES-2108132504 Bernhard HESSE Bernhard Hesse 0000-0001-9106-8980 Bernhard HESSE Bernhard Hesse 0000-0001-9106-8980 Julie VILLANOVA Julie Villanova Jussi-Petteri SUURONEN Jussi-Petteri Suuronen 0000-0002-1632-761X Jussi-Petteri SUURONEN Jussi-Petteri Suuronen 0000-0002-1632-761X Emely BORTEL Emely Bortel Amaia CIPITRIA Amaia Cipitria 0000-0002-9918-1512 Emely BORTEL Emely Bortel Amaia CIPITRIA Amaia Cipitria 0000-0002-9918-1512 Sophie Therese ANUTH Sophie Therese Anuth 0009-0005-3479-1984 Sophie Therese ANUTH Sophie Therese Anuth 0009-0005-3479-1984 Enni MATTERN Enni Mattern 0009-0007-4112-0577 Enni MATTERN Enni Mattern 0009-0007-4112-0577 Example Institute 2028 Data from large facility measurement 2025-05-10T06:00:00Z/2025-05-13T06:00:00Z 2025-05-13T06:00:00Z I CC-BY-4.0 Breast cancer (BC) often metastasizes to bone. Bone tissue is highly dynamic and the cellular and structural microenvironment are strongly interlinked. Mineralized tissue is highly vascularized and rich in osteocytes, highly inter-connected via their osteocyte-lacunar canalicular network (LCN), cell dendrites of few hundreds of nm. Alterations in osteocytes and LCN characteristics have been associated to cancer. With our mouse model of early bone metastasis, we could detect BC cells and small clusters using 3D light sheet fluorescence microscopy (LSFM) and track early bone lesion growth with time-lapse in vivo µCT. Since the LCN plays a fundamental role in cell–cell signaling and bone remodeling, we aim to examine the LCN topology at early stages of metastasis. With synchrotron nanoCT on ID16B we aim to characterize LCN topology as a function of distance to metastatic lesions and BC cells, and provide new insights in the structural and temporal changes during early bone metastasis.